Coding

Part:BBa_K4161503:Design

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


68aa, Aga2 subunit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 268
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 268
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 268
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We expect this sequence to have the ability to express a fused protein containing ipaD antigen on the surface of S. cerevisiae. Therefore, the ipaD gene can be either fused with a signaling sequence targeting the plasmic membrane or a protein that natrually exists on the membrane.

Source

This sequence comes from S. cerevisiae genomic sequence.

References